Triptolide Powder

Triptolide powder, also known as triptolide and triptolide alcohol, is an epoxy diterpene lactone compound extracted from the roots, leaves, flowers and fruits of the Celastrol family plant Tripterygium wilfordii. It is similar to triptolide and triptolide. Alkaloids such as fumenine, tripterygium chemicalbook alkaloid, tripterygium, tripterygium and tripterygium constitute the main active ingredients of tripterygium extract. They are difficult to dissolve in water and easily soluble in methanol and dimethyl. Sulfoxide, absolute ethanol, ethyl acetate, chloroform, etc.

Triptolide is the most active epoxidized diterpene lactone compound isolated from Tripterygium wilfordii. It is one of the main active ingredients of Tripterygium wilfordii. Its relative potency is 100-200 times higher than that of Tripterygium wilfordii total glycosides. times. Pharmacological and clinical trials have shown that it has definite immunosuppressive, anti-inflammatory, anti-fertility and anti-tumor biological activities.

300 kilograms of Tripterygium wilfordii extract medicinal materials (tested, the triptolide content is 0.0043%), crushed to 20 to 30 mesh, put into the extraction tank, add 3 tons of ethyl acetate, then add 360g of ascorbic acid, heat and reflux for extraction 3 times. For 5 hours each time, the extract was filtered and concentrated under reduced pressure to obtain 8.3 kilograms of extract;

Dissolve the extract with 360 liters of methylene chloride and pour it into the extraction tank. Another 17.28 kilograms of NaOH was weighed to prepare 720 liters of 0.6moL/L NaOH solution. Put it into the extraction tank in two batches, wash the dichloromethane solution with alkali twice, and concentrate the dichloromethane phase to obtain 2 kilograms of alkali-washed extract; take 10 kilograms of 200-300 mesh Qingdao marine column chromatography normal phase silica gel, Wet method is used to install a column with a diameter of 35cm. After alkali washing, the extract is loaded and eluted with methylene chloride first. When the color of the eluent turns Chemicalbook light, 2 column volumes are eluted with a mixed solvent of methylene chloride: methanol = 50:1. , then elute with methanol, and concentrate the methanol eluate to obtain 427g of methanol eluate;



Take 20 kilograms of 200-300 mesh Qingdao marine column chromatography normal phase silica gel, wet-install a 50cm diameter column, and load the methanol eluate in sequence Use a mixed solvent of petroleum ether: ethyl acetate = 10:1, 6:1, 4:1, 2:1, 1:1 (volume ratio) for gradient elution. Each ratio elutes about 8 column volumes. The eluate containing triptolide was collected, concentrated to about 1/8 volume and then left to crystallize at 4°C to obtain 13.8g of triptolide crude product;

The crude product was reconstituted at room temperature using isopropyl ether as the crystallization solvent. After crystallization, 12.3g of pure triptolide powder was obtained by filtration, and its purity was 99.4% as detected by HPLC.

Triptolide has rheumatoid treatment, anti-tumor, anti-inflammatory, immunosuppressive, anti-viral and other effects. Triptolide is a diterpenoid triepoxide with immunosuppressive and antitumor properties.

In recent years, a large number of in vivo and in vitro studies have proven that this product has good anti-tumor activity against a variety of cancers, such as leukemia, breast cancer, pancreatic cancer and lung cancer. Recent research in Chemicalbook has found that in addition to specifically affecting certain proteins and signaling pathways, triptolide also inhibits a wide range of gene transcription, but its mechanism of action needs to be further clarified.

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