Cordyceps Militaris Extract Glucan

Cordyceps Militaris Extract Glucan is a natural polysaccharide with β-(1→3) and β-(1→6) glycosidic bonds as the main chain and rich side chains. It is mainly isolated and purified from artificially cultivated Cordyceps militaris fruiting bodies or mycelium. Its molecular weight is usually distributed between 1×10⁴–14×10⁴ Da, and its unique triple helical conformation makes it have higher biological activity than ordinary plant polysaccharides. Since Cordyceps militaris has been included in the list of new resource foods in my country, its glucan, as a safe and stable functional factor, has quickly become popular in the fields of food, health products and medical beauty raw materials, and has become a cost-effective choice to replace Cordyceps sinensis polysaccharide.

Raw material pretreatment: Select dried Cordyceps militaris fruiting bodies and crush them through a 50-mesh sieve.

Ultrasound-assisted water extraction: material-liquid ratio 1:30 (g/mL), 90 ℃ water bath for 3 h, 200 W ultrasound for 60 min during the period, which can significantly improve the dissolution rate of polysaccharides.

Enzymatic impurity removal: Add 2% α-amylase, keep warm at 50 ℃ for 60 min, selectively hydrolyze α-glucan, and reduce the pressure of subsequent purification.

Alcohol precipitation: Concentrate the extract to 1/5 of the original volume, slowly add 95% ethanol to a final ethanol concentration of 80%, stand at 4 ℃ overnight, and collect the precipitate by centrifugation.

Deproteinization and purification: After deproteinization by papain (0.5%, 45℃, 2h) combined with Sevage method, the powder is chromatographed by DEAE-FF ion exchange column and macroporous resin HPD-750, and freeze-dried to obtain Cordyceps militaris glucan powder with purity ≥90%, concentrated molecular weight distribution and complete triple helix structure.